NOVEL CORONAVIRUS RT-PCR DETECTION KIT

Product Specification:

INTENDED USE:

  • This product is intended for the rapid detection of 2019-nCOV in human.
  • Throat swab or sputum samples.
  • Coronavirus is a +ssRNA virus with envelope.
  • Its diameter is about 80-120 nm.
  • Ts genetic material is the largest of all RNA viruses.
  • It is an important pathogen of many livestock, pets, including human diseases, and can cause many kinds of acute and chronic diseases.

PRINCIPLE OF DETECTION

This product is a fluorescent probe-based TaqMan RT-PCR assay system. Firstly, the RNA of 2019-nCov will be reverse transcribed into cDNA by reverse transcriptase, and then PCR amplification will be performed with cDNA as template. During amplification of the template, the TaqMan probe will be degraded due to the 5′-3’ polymerase activity and exonuclease activity of Taq DNA polymerase, then the separation of fluorescent reporter and quencher enable the fluorescent signal to be detected by instrument.
The ORF1ab gene of 2019-nCoV will be detected qualitatively by FAM channel, the N gene of 2019-nCoV will be detected qualitatively by JOE channel, the E gene of 2019-nCoV will be detected qualitatively by ROX channel, and the internal reference will be detected by CY5 channel. dUTP and UNG enzyme are used in the kit to prevent contamination of the amplified products.
Internal Reference is used in the kit for quality control starting from sample collection.

PRODUCT CONTENTS:

Note: Do not mix the components from different batches for detection. The positive control of 2019-nCOV and internal reference was constructed artificially, and they were not infectious.

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